qRT-PCR (quantitative Reverse Transcription Polymerase Chain Reaction) is a cornerstone technique in molecular biology used to measure gene expression with high sensitivity and precision. Our video provides a clear, step-by-step breakdown of the process, from reverse transcription using reverse transcriptase—converting RNA into complementary DNA (cDNA)—to the thermal cycling stages of PCR: denaturation, annealing, and extension. We explain how SYBR Green dye binds to double-stranded DNA, allowing fluorescence to track DNA amplification in real time. The concept of Ct values and how they relate to initial RNA concentration is also covered, alongside the importance of avoiding common errors like incorrect threshold settings or degraded RNA.

To produce this educational content, we used Fliki AI for smooth narration and video creation, ensuring accessibility and engagement. BioRender was employed to generate professional-grade visuals that clarify each phase of the qRT-PCR technique. Our goal was to make this essential technique digestible for students and researchers alike, while highlighting its vital role in both clinical diagnostics—such as COVID-19 testing—and ongoing biomedical research. By combining storytelling with visualization tools, we aimed to bridge the gap between complex science and clear understanding.