The educational video titled “Understanding IC₅₀ Values and Their Analysis Using GraphPad” offers a comprehensive overview of how to determine and interpret IC₅₀ (half-maximal inhibitory concentration) values through nonlinear regression analysis using GraphPad Prism. Designed as an accessible yet scientifically rigorous resource, the video walks viewers through the experimental design, data input, curve fitting, and interpretation of dose-response data—key steps in pharmacological and biochemical research.
Understanding IC₅₀ values is crucial in drug discovery and toxicology, as these metrics quantify the potency of a substance in inhibiting a specific biological or biochemical function. The video emphasizes best practices in data visualization and statistical modeling, enabling researchers to
derive meaningful conclusions about compound efficacy and mechanism of action.
This instructional content was developed using Fliki for video narration and editing, and BioRender for high-quality scientific illustrations, ensuring clarity and visual engagement for diverse audiences, including students, laboratory professionals, and early-career researchers.
Citations
- Created in BioRender. IC50 Example. Cosgrove, J., Coulbourne, F., Reyna, I., Serna-Flores, G. (2025) https://BioRender.com/dzfo9q6
- “Understanding IC50 Values and Their Analysis Using GraphPad.” Fliki AI, uploaded by Cosgrove, J., Coulbourne, F., Reyna, I., Serna-Flores, G., April 21, 2025, https://app.fliki.ai/editor/67c65630018b7329ffa7204b
